SNP Marker Developing And Mapping Of TaDREB1 Gene Related To Drought Resistance In Wheat

It is of great significance to find the polymorphism of functional genes, develop the molecular marker of functional genes and map the subject genes for improving the efficiency of the molecular marker selection in wheat. However, as a hexaploid species, this study was hampered by the complexity of the wheat genome. So, it is also importantly siginificant in theory to study the development of SNP marker in common wheat.TaDREB1 transcription factor is up-expressed regulated proteins, which is induced by much of obiotic stress and play an inprotant role during the carsscas of gene expressional regulation when wheat faced with stresses. Therefore, it is of greatly practical significance to develop SNP marker of TaDREB1 gene and map it finly, not only for the study of genetic mechanism of drought-tolerance, but also for the genetic improvement of new cultivars of wheat.In this study, the TaDREB1 gene sequences were isolated from four hexaploid wheat cultivars, three diploid relative-species and one tetraploid relative-species. Then, genome-specific primer sets were designed and. three SNPs were detected by sequencing. Finally, a stable SNP molecular marker was designed. We aimed to explore the feasible way to develop SNP molecular marker of TaDREB1 in wheat, densify the molecular genetic linkage map and provide a novel way for mining and exploiting wheat germplasm fully. The main results were as follow:1. A series of genome-specific primers of TaDREB1 were designed: P18, P20, P22, P21 and P25. Using these genome-specific primers, the sequence of TaDREB1 was islated from each genome of common wheat, wheat tetraploid relative-specises and wheat diploid relative-specises. Then, the sequences from A, B and D genome of common wheat were submitted on GenBank, accession numbers were: DQ195068, DQ195069 and DQ195070.2. Three SNPs: S608, S705 and S732, were detected among four hexaploid wheat cultivars.