Melampsora Larici-Populina DNA Polymorphism And Isozyme Analysis

Melampsora larici-populina Kleb. Also Called the leaf rust of populus cathayana, Widely distributes in various poplar transplants of the world, may do harm to many kinds of poplars composed of Tacamabaca,Aigeros,hybridized popular and Diversiform-leaved poplar,it is the most Serious disease and the spread range is also the most.The paper is to research physiological differentiation and DNA polymorphism of MLP。The main result is as follows:1.Investigating the Poplar Resistance: the difference of sensitivity is prominent。Other poplars are infected by Melampsora larici-populina in different degree, except that Populus deltoids and Populus deltoides×P. trichcarpa are immune,The results indicated that their resistance abilities : Aigeros > Aigeros×Aigeros > Aigeros×Tacamabaca > Tacamabaca。2. Analyzing the DNA Polymorphism: 14 random primers were selected to analyze the DNA polymorphism of 13 species of Melampsora larici-populina, the result indicates: The isolates of MLP have resemblance and difference, thee differences reflect the DNA Polymorphism of them。But the Polymorphism is not related to their origin.the result is consistent with departed one.In the lever of DNA,13 isolates of Melampsora larici-populina are divided into four groups:the first is composed of GL and CJQ,the second consists of Ts,Tt,Scb and Nm, the third contains Ha,Hf,H159,ZST,Bqy and Hdao,the last is Qh.3. Analyzing MLP in isozymes level: three kinds of isozymes(Esterase,Peroxidase,Catalase)all can reflect the physiological differentiation of MLP,and the differentiation is more obvious,but not interrelated with their origin,in addition to,the patterns of Esterase are better。In the lever of isozyme,10 species of Melampsora larici-populina are divided into four groups:the first includes Nm ,GL, H159,CJQ and ZST ;the second is Qh and Bqy, the third is Scb and Ts, Tt is included in the forth group。The result is identical basally with past one about pathogenicity mensuration。Above studies will provide a reliable means for latter study of physiological division , establish stable fundation for further study of MLP and controlling effectively the disease。...