The Analysis Of Genetic Structure Of A Cultured Half-smooth Tongue-soles Population And The Isolation Of Sex-specific AFLP Markers In Tongue Sole

Half-smooth tongue-sole is a commercially important cultured marine fish in China. It grows fast, is big in size and nourishing. However, recently the wild source of half-smooth tongue-sole is decreasing year by year because of excessive fishing, environment pollution, which has been paid attention to. More and more culturists begin to culture the half-smooth tongue-sole. AFLP technique was used to screen the genome DNA of cultured population and analyze the genetic diversity in order to do something for the breeding of half-smooth tongue-sole. Besides, the female individuals of half-smooth tongue- sole grow 2-3 times faster the male ones. A mature female half-smooth tongue-sole is 820 mm in size, but a male individual is only 420 mm. Thus, the development of all-female stock would be of significant benefit for aquaculture. In the present study, AFLP technique was used to identify the sex-specific markers in half-smooth tongue-sole. Some results were obtained as following:1. 14 primer-combinations were used to screen the genome DNA of 30 individuals of half-smooth tongue-sole cultured population. There were a total of 1145 AFLP loci detected. The average percentage of polymorphic loci is 62.20% and the gene diversity of the population is 0.1968. This showed that the culturing of half-smooth tongu-sole was in a good condition.2. 64 primer-combinations were used to screen the genome DNA of tongue-soles. Only 4 primer-combinations amplified 7 female-specific markers. The relevant AFLP bands were marked and excised, reamplified, cloned and sequenced. For the AFLP marker M1, a pair of PCR primers were designed, which could amplify...