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Primary Study On Reproductive Biology Of Tetraploid Pacific Oyster (Crassostrea Gigas Thunberg)

Posted on:2007-06-28Degree:MasterType:Thesis
Country:ChinaCandidate:K T ShiFull Text:PDF
GTID:2143360185490604Subject:Aquaculture
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Contrasting to the diploid Pacific oyster, this paper is focus on the teraploid Pacific oyster. The study deals with the reproductive biology of tetraploid Pacific oyster by reaserching the survival time of spermatozoon which leaving body, the egg amount, the egg diameter, sex rate and embryonic development. Meanwhile the author used he method that self-reproduction in tetraploid Pacific oyster to build the tetraploid population. And the author observed the tetraploid Pacific oyster sperms entry into the diploid Pacific oyster eggs by scanning electron microscoic. It proves the feasibility which to hybridize. This paper supplys the base on the experiment and practice,and richs the breeding theory of the polyploidy.The main contents consist of the following four parts.1. Reproductive behaviour of tetraploid oyster reaserchThis paper reaserched the tetraploid Pacific oyster on the amount and the dimeter of egg, the sex rate and embryonic development in laoshan and haiyang of shangdong provice. The study indicates that the egg amount of shell length 3~4cm of tetraploid oyster is range from 1.87×105~1.67×106(averge 35.73×106). The polyploidy have larger egg dimeter than the commons. The dimeter of eggs in tetraploid is 64.58μm, 14.29% larger than triploid (55.72μm) and 31.06% larger than diploid (51.28μm). The sex rate is 1∶24 (♀∶♂) from one-year-old teraploid, but it is 38.46% in two-year-old one. The tetraploid oyster was found the first polar after 12 min, and a little faster than the control groups. But there are no distinct difference among of tetraploid, triploid and diploid on their embryonic development.2. Comparison on survival time of spermatozoon between diploid and tetraploid Pacific oyster, C. gigsa ThunbergThe survival capacity of sperm from diploid (2n) and tetraploid (4n) Pacific Oyster in different temperature and density was compared in this study. Results showed that survival time of sperm from both 2n and 4n shortened with the increased...
Keywords/Search Tags:Crassostrea gigas, Tetraploid, Reproduction Biology
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