Genetic Diversity Study Of Adelphocoris Lineolatus (Goeze) From Inner Mongolia Based On Cyt B Gene Sequeces

Adelphocoris lineolatus ( Goeze) is a kind of very important agricultural pest in the Palearctic region. At the 50's to 60's in 20 centuries, it once harm to the yield of our country cotton seriously, and for this reason, it is always valued by entomological researchers. Entomological researchers devote into morphological, anatomical and biological research in order to control this pest for long period of time. In the morpological researching, it can be seen that among different individuals, different populations, there have differences in the somatotype size, body colour and mottling.In this study, we selected mitochondrial DNA(mtDNA) cytochrome b(Cytb) gene as molecular marker, amplified 433bp sequences segment with a pair of universal primers for 11 populations of Adelphocoris lineolatus ( Goeze). All gained sequences were aligned using software Clustalx1.8, DNAStar, and molecular phylogenetic tree were constructed with commonly used methods of N-J, ME and MP by using software MEGA2.0. Interpreting the reason of genetic diversity, populations genetic structure, distribution regularity among and in populations from molecular level in order to provide theoretical basis for control Adelphocoris lineolatus ( Goeze). It also provide valuable basis datum in inheritance and variation, evolutionary potential, the mechanism of pesticide resistance, utility beneficial gene,control harmful gene and co-evolution study.We can draw conclusions as follows through analysising these results:1.Using the method of SDS-Proteinase K, we can extract enough genome DNA for PCR, but the extractive of dry specimens can not be amplify sufficiently.