The Study On Differential Expression Of Genes Of ABA Biosynthesis Key Enzymes During The Root Signal Period In Winter Wheat

Root sourced signaling is the pre-alerting mechanisms of plants to drought and can make plants taking a series of reactions such as growth, physiological, biochemical and molecular responsiveness to survive the drought-stress. Two winter wheats, Triticum aestivum L.cv'changwu135'and Triticum aestivum L.cv'pingliang40'were mono-cultured and mix-cultured in the greenhouse. The aims were to find out the variation of non-hydraulic root signal in the progressive soil drying and the mRNA difference of the expressing quantity of ABA biosynthesis enzymes, 9-cis-epoxycarotenoid dioxigenase (NCED) and abscisic aldehyde oxidase(AO). The stable expressingβ-action was used as reference in the semi-quantitative RT-PCR technique.The main results of the study were as follows:1. No matter monocultures or mixed-cultures, the amplitude of the soil water contents during the root signaling period of the root-excised winter wheat(secondary root cutting) are wider than that of the without root excision. The soil relative water contents (RWC) at the appearance of root signals of wheat after root excision is 58.3%, the soil relative water contents (RWC) is 48.2% when root signal shifts from the non-hydraulic to the hydraulic .The width of RWC is 10.1% during the non-hydraulic root sourced signaling period. The three parameters of the winter wheat without root excision are 54.9%, 48.9% and 6%, respectively. After root excision, RWC of the winter wheat was obviously enlarged, that is to say, the duration of the non-hydraulic root sourced signaling was prolonged. The width of the soil water contents during the root signaling period of the'changwu135'was obviously...