| White-coated meat was favored by consumers, and the selection for white coat-color domestic pigs was started since medieval times through which the pigment spots are usually eliminated from breeding herds. Unfortunately, breeders have not been able to completely fix the desired phenotype so far in spite of nearly 100-year strong selection due to the diversity of recessive alleles of pigment spots and the complexity of coat-color forming. With the speedy development of molecular biology and biotechnologies, researchers have been studying the genetic characters of coat color on the level of molecule. For the purpose of studying the polymorphisms of KIT gene and quantification of the ratio of wild-type/mutant nucleotide at the splice site and exploring the relationship between KIT gene and Rongchang pig coat color, this study was conducted to apply single strand conformation polymorphism analysis of polymearase chain reaction products (PCR-SSCP) and pyrosequencing in Rongchang pigs (including white and other coat color), Duroc, Landrace and Large White pigs, and chose KIT gene of mast/stem cell growth factor receptor (MGF) as candidate gene.The results of polymorphisms of KIT gene showed that the genotypes of KIT gene were specific among the breeds. Electrophoresis patterns of PCR-SSCP for intron 17/18 of porcine KIT gene showed that the same breed detected individuals have the same strips of KIT gene; Landrace and Large White pig have an identical strips of KIT gene (4 strips) and Rongchang pig and Duroc have another identical strips of KIT gene(2 strips). KIT gene in pigs have different strips was sequenced. The result of sequencing KIT gene in a Rongchang pig showed that the mutations of KIT gene of Landrace and Large White pig (G→A and the deletion of AGTT) were not found in Rongchang Pig with the similar coat color, which was against that white coat color was controlled by traditional dominated white allele gene. Therefore, the conclusion was attained that the...
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