| When pathogen invasion, the natural immunity is the multicellular organism rapid control infection first defense line, in the vertebrate, participates in this kind of early immune response each kind of cell and the member has the decisive function to its acquired immunity formation. The research discovered that, the mammal Toll acceptor family belongs to the cell factor acceptor or the pattern recognition acceptor, can similar activate but the quite same not less than signal extension to lead the way, thus displays the natural immunity and the acquired immunity control action, by resists the external microorganism or parasite's infection.In this research, the lymphocyte separated pig spleen and the lymph node ion phytohemagglutinin(PHA) and the lipopolysaccharid(eLPS) stimulates together by the plant haemagglutinin from, withdraws total RNA, uses the RT-PCR technology, in domestic by partitions for the first time the clone law, respectively clones outside the pig TLR9 gene butcher in the area, the butcher the area sequence, the fragment size respectively is 1820bp, 1700bp. Outside the pig TLR9 gene butcher clones which through the sequence analysis in the area, the butcher on the area sequence nucleotide sequence and the amino acid sequence and the GenBank issue sequence homology respectively is 98% and 100%. This research choice has the important function outside butcher area gene fragment, Take reorganizes material particle pGEM-TLR9-1 as the template carries on expands increases, after expands the production increase and the pGEX-4T-1 connection transforms the backwoods coli, cuts, PCR after the enzyme and measured the foreword appraisal, induces the expression with ITPG, swims after the SDS-PAGE electricity carries on the analysis, indicated, obtains the reorganization protein is the goal protein, its molecular weight is approximately 67KD, the expression quantity occupies the mycelium total protein about 20%, explained already...
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