| Summary: Avian Influenza(AI)was a highly contagious disease caused by type A influenza virus. It was known that AIV can attack human and cause human death after the outbreak of AIV in Hong Kong in 1997.Starting in 2004, an epidemic of highly pathogenic avian influenza(HPAI)have taken place in south-east Asia. Human infection case and death number were raised. It was indicate that HPAI can not only do harm to poultry, but also have potential threat to human health. Therefore, It was essential to study the characteristic of HPAIV in order to know about the molecular basis of virus evolution and the relationship between structure and function of H5N1 subtype avian influenza viruses.A/Duck/Guangxi/53/02 is a H5N1 subtype avian influenza virus isolated in healthy ducks from south China. Full gene sequence analysis of A/Duck/Guangxi/53/02 showed that there are 6 continued basic amino acid (RRRKKR) in the cleavage site of HA, which is the characteristic of highly pathogenic avian influenza viruses .The glycosylation site is at 26,27,39,170,181,302,500,559. The amino acids at 226 and 228 site of HA are Gln and Gly, which is the property of avian origin influenza virus.The virus has 19 nucleotides deletion from 49 to 68 of the neck of NA. The glycosylation is at 50,58,63,68,88,146,235,386.It has 15 nucleotides deletion in NS. The amino acids at 627 and 701 site of PB2 gene are Glu and Asp respectively. In spite of the high homology of HA gene of DKGX/53/02 and HK/483/97, the homology of other genes between DKGX/53/02 and HK/483/97 were low. It indicated that the DKGX/53/02 has different origin with HK/483/97.The homology of HA,NA,PB1,NP,M gene between DKGX/53/02 and GSGD/1/96 is above 97%,The homology of all gene segment between DKGX/53/02,DKGJ/13/02 and DKSH/35/02 is all above 90%,These viruses are highly pathogenic to chickens, but showed different pathogenicity to Balb/c mice. GSGD/1/96 was non...
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