Establishment Of PCR Detecting Method Of Eperythrozoon Suis

The Eperythrozoonosis suis was produced by Eperythrozoon suis which parasite on erythrocyte surface and free in blood plasma and marrow of swine, which have symptom of piglets febrile, anemia, jaundice and origin abortion. This disease always happened with other disease of swine. It was difficult to prevention and cure in clinic. So it took a great of economic losses to raise pigs. Traditional methods of final diagnosis principal have: blood smear, staining by microscopic examination and microscopic examination directly in morphology diagnosis. In serology detection, there were complement fixation test, fluorescent antibody test, IH and so on. But the specificity, sensitivity and accurate were lower in the methods just mentioned. The modernization produces to need to be building up a kind of fast, accurate, examine a method efficiently.The experiment analyzed the homology of gene sequence of Eperythrozoon suis what were reported in GenBank, to select its conserved sequence as amplification district. To design and screening a pair of suitable PCR primers with Oligo6.0 and Primer Premier5.0 software, the amplification fragment were 541bp, and cloned into pGEM-T Easy Vector system, construct a recombinant plasmid containing Eperythrozoon suis gene fragment, and it was identified by Not I restriction enzyme, PCR and sequence analysis. It demonstrated that the cloned gene were Eperythrozoon suis specificity gene. Initial to establish PCR detection method for Eperythrozoonosis. The clone of Eperythrozoon suis sequence compared with E.suis-GuangDong (AY492086), the results show that the homology is 100%, compared with E.suis-USA (U88565) the homology is 97%. Proved by specificity and sensitivity experiment, this diagnosis sequence could not amplify Toxoplasma,...