Study On Genetic Diversity Of Natural Populations Of Oligostachyum Scabriflorum

Genetic diversity and population genetic structure of natural populations of Oligostachyum scabriflorum from Yongan City,China were studied at phenotypic and DNA levels.The relationships and gene flow between these populations were discussed.Based on comprehensive analysis.the current situation of the genetic resource of Oligostachyum scabriflorum was evaluated.and some suggestions were refered.The results are as follows:1.The phenotypic variation was discussed with thirteen phenotypic traits analyzed through correspondence analysis, nested variance analysis and differentiation coefficient analysis.The variation within subpopulations was much greater than that among subpopulations,but phenotypic differentiation coefficient V_ST =37.29%,the differentiation among the subpopulations was great.The correlation between the thirteen phenotypic traits and the little envirenment factors was discussed.For instance, the leaf phenotypic traits were remarkably correlated to altitude;the height of bamboo was correlated to altitude;all of these phenotypic traits were not correlated to the gradient and the slope;clear height and nodule length at breast height were correlated to the site type; the height of bamboo was correlated to arbor type.Throught cluster analysis.the Qingshui's subpopulations were all divided into the same subdivision,and the Ansha's subpopulation A5 was divided into the Hongtian's population.and the Hongtian's subpopulations were divided into two groups.It was maybe the result from human's frequent disturbance.2.The improved CTAB method secceedly exacted and puried the total DNA from the dried leaves of Oligostachyum scabriflorum and the DNA was absolutely meeting the RAPD's request.3. The reliable RAPD analysis system was established. The reaction volume is 20ul and reaction mixture consist with 1ng/ul DNA, 0.1mM of dNTPs, 2.0mM Mg²⁺ , 0.5uM random primer and 1U Taq polymerase. RAPD program is 2 minutes at 94 ℃ for predenaturation, then 38 cycles of 30 seconds at 94℃ for denaturation, of 30 seconds at 38℃ for annealing, of 90 seconds at 72℃ for extension, finally extension at 72℃ for 7min, stopped...