Genetic Analysis Of T-DNA-inserted Mutant In Indica Rice

With Arabidopsis, rice,and other models throughout the plant genome sequence determination completed,a large number of new or unknown functional genes should be identified.Mutant was a most direct and effective tool in identifying gene function. Therefore,model plant mutant pools, especially T-DNA-inserted mutant pool would be the commonly used research strategy for analysing gene function.Constructed of plant expression vetctor carrying modified GFPgene(SmGFP5) of non-promotor were introduced into rice Minghui 86(Indica Sativa L.) mediated by Agrobacterium in the present experiment.We had obtained 9200 independent clones' transgenic plants.PCR analysis of 93.4% transgenic plants showed that exogenous genes were integrated into rice genome.We investigated phenotype characterization of 1329 T₁ generation of transgene Indica. We found that the types of phenotype variation is rather more and obtained 279 clone of phenotype variation,which the frequency of phenotype variation is 21.0%.The mutation phenotype was related to 9 kinds of trait such as leaf, plant height,tiller,panicle,flower organ,grain shape,fertility,heading date and lethal,and so on.Moreover,about 12.9% transgenic plants appeared stolen green segregation in seedling stage such as etiolation and albino. Among them,about 71% stolen-green seedlings largely died before the period of three-leaf;while about 29% clone largely turned green later period. At the same time we genetically analyse an important application of production prospects of early-maturing mutant.In addition,53 flanking sequences at T-DNA-inserted site of the part T₀ transformants were obtained.Homologous comparison by BLASTn was conducted in GeneBank.We found 33 had good identify to rice genome sequence,the others were vector backbone sequence.