The Effect Of Materials On The IVM Of Bovine Oocytes

In Vitro Maturation of oocytes is the basic of in vitro culture embryos, which can guarantee us more quality oocytes for in vitro fertilization and embryos transfer. Although the technology of In Vitro Maturation is more and more maturated, the mechanism of maturation needs further study. This thesis compared the effect of Uracil, ITS, and ATP on the maturation of bovine oocytes and further development, observed the microstructure changes of oocytes and parthenogenetic embryos among different groups, and granule cell apoptosis among different groups. The main point is to illustrate the effect of Uracil, ITS, and ATP on bovine oocytes'maturation.1. In bovine oocytes'IVM research the solution was divided into three groups: basic solution as the control group (CG), basic solution supplied with 50μg/mL Uracil and 10μL/mL ITS is the first group (FG), and basic solution added by 0.67 mg/mL ATP is the second group (SG). The result shows the rate of oocytes with granule cells full amplified in FG (87.31%) is significant higher than SG (70.54%) (P<0.05), and CG (68.67%) (P<0.01). This rate of SG is higher than CG, but not significant higher (P>0.05). The rate of maturation, cleavage, and 8~16 cell of FG (72.65%, 55.56%, and 35.56%) are significant higher than CG (57.92%, 43.88%, and 24.59%) (P<0.01). Although the maturation rate of SG (60.39%) is not significant higher than CG (57.92%) (P>0.05), the cleavage and 8~16 cell rate is significant higher than CG (P<0.05). The rate of maturation and cleavage are significant difference between FG and SG (P<0.05), however, there is not significant difference in 8~16 cell rate (P>0.05). The rate of bovine oocytes stained by JC-1 in FG (51.11%) is significant higher than SG (40.00%) (P<0.05), and CG (33.33%) (P<0.01). There is no significant difference between FG and SG. Uracil and ITS can proliferiate the granule cells, improve the maturation rate, cleavage rate, and 8~16 cells rate, and enhance the mitochondria's activity.2. The results of electron microscope which observes maturated oocytes and pathenogenetic embryos show the changes of granule cells are not significant among three groups, the mitochondria'double deck of oocytes and pethnogenetic embryos are not clearly, and full of the vacuole denaturalization. The mitochondria are more abundant and its electron desity in FG is more closed to cytoplasm than other groups. We abserve the yalk granules are...