Genetic Structure And Genetic Diversity Of Two Sparidae Species In Chinese Coastal Waters

To assessing genetic structure and genetic diversity of yellowfin seabream (Acanthopagrus latus) in coastal waters of China, we compared mtDNA control region sequences from three localities (ShenZhen, n=40; XiaMen, n=31; and BeiHai, n=29) furthermore, we also obtained 22 Acanthopagrus latus D-loop sequences from GeneBank (AF549503-AF549524) for analyzing together. There are 106 polymorphic sites and 10 insert/deletions in 427bp D-loop sequence that defined 108 haplotypes, high level of genetic diversity were found among the 122 D-loop sequences (h=0.9929-0.9952, p=0.0125-0.0187). Constructing molecular phylogenetic tree of the three localities by using UPGMA and NJ methods based on Nei's genetic distance, the three populations clustered two branches, one is BeiHai (BH) population, and the other is another two populations XiaMen (XM) and ShenZhen (SZ). Significant genetic differentiation between (BH) and other two populations (XM and SZ) was found by analysis of molecular variance (AMOVA) (BH and XM: Fst=0.5483 p<0.001; BH and SZ: Fst=0.5609 p<0.001), moreover, genetic differentiation between population XM and SZ was not significant (Fst=-0.0023, p>0.05); the level of gene flow was estimated by using the equation of Wright Nm= (1/Fst-1)/2, low level of gene flow was found between population BH and other two populations (SZ=0.3914, XM=0.4119), whereas the Nm value between SZ and XM was 204.09 which indicated gene flow between population SZ and XM was more free and consequently larger. Based on our study, we can conclude that the three wild populations of yellowfin seabream (Acanthopagrus latus) in coastal waters of China belong to two populations, one is the popultions SZ and XM, the other population is BH population.536bp mitochondrial D-loop gene segments of Crimson Tai (Evynnis cardinalis) collected in Chinese costal waters from XiaMen and QuanZhou (Fujian province), ZhouShan (ZheJiang province), ShenZhen (GuangDong province), BeiHai (GuangXi province), SanYa (HaiNan province) were amplified by PCR technique and sequenced. High genetic polymorphism in the six localities was examined. 91 polymorphic sites (16.98%) and 5 insert/deletions were found in these sequences that defined 191...