RAPD And Diosgenin Contents Analysis Of Dioscorea Zingiberensis Populations

Dioscorea zingiberensis is unique to our country. The need of Dioscorea zingiberensis for people is getting more and more because of the great medical value of diosgenin that it contains. But the research of Dioscorea zingiberensis resources is rare.In this study, we used RAPD marker to estimate the genetic diversities of 15 Dioscorea populations and tested the contents of diosgenin of 23 Dioscorea zingiberensis plants.1. RAPD were applied to detect genetic diversity of bulking samples of 11 Dioscorea zingiberensis populations and 4 other Dioscorea populations from Dioscorea resources plot of Huazhong Agricultural University, The differences of amplified DNA bands in these varieties are reflection of their differences in genetic background. For the 11 Dioscorea zingiberensis populations, 19 RAPD primers produced 153 bands, out of which 113 bands were polymorphic. The polymorphic bands accounted for 73.86% of the total amplified bands. The Nei's association coefficients and genetic distances of all populations were calcultated respectively and their UPGMA (Un-weighted Pair Group Mathematics Average) relationship tree were established. By clustering analysis and correspondence analysis, the relationships and genetic differences among these populations were displayed.2. The results of the diosgenin contents of 23 Dioscorea zingiberensis plants showed that most materials from the same area have little difference in diosgenin content, however those materials have significant difference in diosgenin content show obvious difference of water content, water is important for diosgenin accumulation, and extra low water content is obviously linked with low diosgenin content, water content does have obvious linear relationship with diosgenin content.3. We believed that Dioscorea parviflora from Yun nan province had obvious genetic variant with the Dioscorea zingiberensis and it could be used in Dioscorea...