Cloning And Characterization Of Four Genes Related With Fiber Development In Cotton (Gossypium Hirsutum L.)

Cotton fibers are important textile materials that play an important role in national economy and people's lives. Each cotton fiber is a single cell of ovule epidermis that elongates to 2.5-3.0 cm within approximately 16 days post anthesis (DPA). Fibers cells are the longest cells in planta. Scientists are devoted to elucidate the mechanism controlling fiber elongation. Within the development of cDNA array, people can analyze many genes in one experiment and clone unknown genes. The wild line (li1) and the Ligon lintless mutant line (Li1) segregated from the self-crossing progenies of the Ligon lintless mutant line(li1) are different in the vegetable phenotype. The distinct difference between them is the development of fiber. Using the two types of plants as material, we isolated genes preferentially expressed in rapidly elongating fiber cells with genechips to provide the information of fiber elongation.Four up-regulation expression cDNA clones were separated from developmentally different cotton fiber library of elite quality material 7235 library by genechips of the 10DPA period between the wild line (li1) and the Ligon lintless mutant line (Li1) segregated from the self-crossing progenies of the Ligon lintless mutant line(li1): the coding product of GhVP1(H~+-pyrophosphatase) was 667amino acids that showed 79% identity with AVP1 in Arabidopsis; the coding product of GhGAD(glutamate decarboxylase) was 542 amino acids that showed 92% identity with glutamate decarboxylase isozyme 3 in Nicotiana tabacum; the coding product of GhAGP(Arabinogalactan-protein) was 63 amino acids that showed 52% identity with AGP15 in Arabidopsis; the coding product of GhFUCT(alphal1,3/4-fucosyltransferase) was 406 amino acids that showed 69% identity with FUT13 in Arabidopsis.Subsequent RT-PCR analysis of the four genes indicated: the expression quantity of the all four genes were more in the wild Iine(li1) than that in the Ligon lintless mutant line(Li1) at 10DPA, which were consistent with the result from genechips; Judging from the...