Chemical Synthesis Of RSOD Gene And Its Transformation For Apple

The molecular breeding of plants that have been genetically engineered for improved disease,pest ,weed resistance and abiotic stress tolerlance has been undertaken with the goal of improving food production. More recently, it has been realized that transgenic plants can serve as bioreactors for the proteins or compounds which have important industrial or clinical applications. Several different recombinant enzymes and antibodies have been produced in this manner. From an economical point of view, the production cost of biomaterials using plants is less than that for compounds produced through fermentation. For plants, solar energy, CO₂ and inorgannic chemicals are the only materials needed once suitable seeds have been produced, but equipment,substrate,and electric energy are constantly needed for materials produced by fermentation. Attention is now shifting from basic research towards commercial exploitation, and plant bioreactor is reaching the stage at which it could challenge established production technologies that use bacteria, yeast and cultured mammalian cells.Human SOD which used as a pharmaceutical agent or cosmetic ingredient is widely useful and has significant commercial value.In this paper, Firstly, we modified human Cu/Zn-SOD gene according to the plant codon usage, the new human Cu/Zn-SOD gene contains tobacco PR-S signal in the N-terminal and KDEL ER retension signal at the C terminus.Then the plant binary vectors containing the new human Cu/Zn-SOD genes which were controlled by CAMV35S promoter or fruit-specific PG gene promoter were constructed,they were transformed into Arabidopsis and apple plants using Agrobacterium-mediated transformation.Twenty-first transgenic Arabidopsis plants were obtained after grobacterium-mediated transformation. GUS-staining and PCR analysis confirmed that the new human Cu/Zn-SOD gene had integrated into the Arabidopsis genome, semi-quantitative RT-PCR revealed that the new human Cu/Zn-SOD gene was transcripted in Arabidopsis plants, and test of kanamycin torlerance of T₁ seedings demonstrated that the tolerant and sensitive progenies fit a 3:1 segregation ratio,transgenic plants showed higher level of salt resistance than that...