The Preparation And Study Of Bovine Anti-Helicobacter Pylori Antibodies

This experiment examined bovine anti-helicobacter pylori antibodies preparation methods, immunized the cows with Helicobacter pylori(Hp) whole-cell antigen (4×10~9CFU/head). Cows were immunized with Hp vaccine via intramuscular injection to the neck. Collect the milk after immune. The titre of anti-Hp in immune serum and milk detected by indirect ELISA showered the immune method can cause immune response efficiently. In the whole milking days, the titre in immune milk keep the higher P/N value.To establish mouse models of Hp infection. C57BL/6 strain mouse, used as an experiment animal, was inoculated orally H. pylori NCTC11637. Inoculated mouse examined by urease test and bacteria cultivation. Bacteria DNA from gastric tissue analyzed by PCR . RESULTS: Hp NCTC11637 can colonize successfully the mouse stomach.Prevention and therapy study of bovine IgG against Hp infection in mouse model: In vitro, in a different pH value of immunization stomach proteinase processing milk, reuse indirect ELISA testing its effect on antibody. In vivo, using immunization milk processing mouse models, observation immunization prevention and therapeutic efficacy of milk. Results in the context of a certain pH value of IgG-Hp reactive stomach proteinase no significant impact, including immunization against Hp-IgG field setting Federal milk to prevent, reduce Hp infection, and it has some obvious prevention and the role of Hp infection C57BL/6 mouse of a certain treatment.IgG-Hp oral arising from the effects of passive immunization is expected to make its application to various Federal health or food related stomach trouble of anti-bacterial immunization treatment.