Study On The Transformation Of Potato With Peanut Resveratrol Synthase Gene Mediated By Agrobacterium Tumefaciens

The expression vector pB3RS with the peanut resveratrol synthase gene (Genbank AY170347) driven by CaMV35S promoter was constructed on the basis of pCAMBIA1301 and identified by PCR and enzyme digestion. The vector pB3RS was transferred into the Agrobacterium tumefaciens strain LBA4404 by electro-transformation and identified by PCR and enzyme digestion. The optimal redifferentiation medium for explants of virus-free potato tubers of "Kexin 3" was MS + ZT 2.0 mg/L + IAA 1.0 mg/L. The callus induction rate on the medium was 100% and the adventitious shoot redifferentiation rate 80%. The test showed that the pre-culture of explants of virus-free potato tubers of "Kexin 3" was not necessary. Using OD₆₀₀ 0.1-0.5 solutions of A. tumefaciens LBA4404/pB3RS, submerging explants in bacterium solutions for 5-10 min and co-culturing explants for 2d with the bacteria was suitable for genetic transformation of "Kexin 3". 10 potato clones showed a fragment identical with RS gene in PCR analysis. One of them, "Kexin 3-2" clone, was selected for southern blot analysis and the result was positive in the clone but negative in the control.