Development And Biological Characteristics Of Monoclonal Antibodies Against GP5,M And N Protein Of Porcine Reproductive And Respirtory Syndrome Virus

Clinical manifestations of the Porcine reproductive and respiratory syndrome (PRRS) are characterized by reproductive failure in sows and gilts, pneumonia in grower/finisher pigs, and increased morbidity and mortality in preweaning pigs. The PRRS virus is a member of the Arteriviridae. Other viruses of this family include lactate dehydrogenase-elevating virus (LDV) of mice, equine arteritis virus (EAV) and simian hemorrhagic fever virus (SHFV). Both genetic and antigenic differences have been demonstrated between European and NA PRRS virus. The genome is a positive sense, 15 kb single strand of RNA consisting of 9 open reading frames (ORFs) .The ORFs la and lb of PRRS virus encode nonstructural proteins associated with viral replicase activity. The ORFs 2 through 7 encode structural proteins with molecular masses of 30, 45, 31, 25,19 and 15 kD respectively, which are designated GP2a, GP2b, GP3, GP4, GP5, M and N respectively.Two hybridoma lines producing monoclonal antibody against PRRSV N protein have been established. After the immunization of BALB/c mice with purified PRRSV, the stimulated splenocytes were fused with SP2/0 myelomas to produce hybridomas. The hybridoma lines, designated 4B8 and 4D8. The ELISA titers of cultural cell supernatant of them were 1:32~1:128. The EFA showed the McAbs reacted with cells infected by PRRSV positively. The results of ELISA to purified PRRSV and HIS-N protein indicated that the McAbs were against N protein of PRRSV.Three hybridoma lines producing monoclonal antibody against PRRSV N protein have been established. After the immunization of BALB/c mice with HIS-N protein expressed in...