Molecular Epidemicology And Biological Property Of Newcastle Disease Virus Isolated From Diffrenet Hosts In Jiangsu And Areas Hereabout

Newcastle disease (ND) is a highly fatal and contagious avian disease caused by Newcastle disease virus(NDV). It is also one of the disease as listed A animal infectious disease which causes sever avian epidemic worldwide every year. Because of a negative-sense single-stranded RNA and higher variability, the pathogenic power of NDV isolated from different hosts strengthens significantly in resent years.Recently three researching methods such as monoclonal antibody, restriction endonuclease, amplification of the special function segment of F gene and drawing the phylogenetic tree have been used to study the molecular epidemicology of NDV. To protect different hosts from NDV, it is important to research on molecular epidemicology of NDV widely and choose a vaccine strain with better immunogenicity. Seven of twelve NDV strains isolated from different hosts such as silkie, peafowl, goose, duck and chicken in Jiangsu and areas hereabout were tested by sequence analysis, the biological property and the immunogenicity.The important functional region of the fusion gene of 7 Newcastle Disease virus(NDV) isolates, two from chickens, one from silkie, one from goose, one from peafowl, and one from duck, were amplified by RT-PCR. By the deduced amino-acid sequence analysis and the genotyping characteristics, the results showed that six of seven isolated viruses belonged to NDV genotype VII. The NDV homogenecity between isolated viruses and the standard velogenic F₄₈E₈ was less than 90%, but the homogenecity of isolated viruses were up to 94% compared to that of Taiwan95. It is implied that the currently popular strains of NDV are originated from Taiwan. 6 isolated NDV including isolates from goose and duck were velogenic.It is implied that the pathogenic power of NDV was gradually strengthened. NDV genotype VII are dominating in the prevalence in Jiangsu and areas hereabout, NDV was originated from Taiwan and the pathogenic power are gradually strengthened.To understand the biological property, haemagglutination test (HA), dehaemagglutination test, intravenous pathogenicity index (IVPI), intracerebral...