Studying On Complete Genome Sequencing Of Goose-host Paramyxovirus Strain NA-1 And Coexpression Of F And HN Gene In Eucaryotic Cell

Avian Paramyxovirus consisted of nine serotypes, APMV 1~9, but APMV-1 only included Newcastle disease virus. There existed tremendous virulence diversity among different hosts in which chicken was the most sensitive, pigeon and parrot also could be infected and falled ill. Waterfowls such as geese or ducks constantly were not considered to be infected by NDV. For the past few years, uninterruptly expanding of the host infection spectrum about NDV, chicken, pigeon, parrot and some rare birds could be infected, as well as waterfowls. The outbreak of goose-host paramyxovirus disease broken traditionary theory which said waterfowls could not be infected by NDV. Several studying results indicated that the genome of NDV evolved at an integrity level.Goose-host paramyxovirus strain NA-1 was separated and purified in our laboratory. Complete RNA genome of virus was extracted and the genome nucleotide sequence of goose-host paramyxovirus strain ZJ1 in Genbank was refered to design eight pairs of specific primers, and then eight gene fragments were amplified by the method of RT-PCR. It indicated that the number of complete genome nucleotides from goose-host paramyxovirus strain NA-1 was 15 192nt which contained six nucleotides...