Isolation, Identification And Degradation Characteristics Of A Phenazine-1-Carboxylic Acid (PCA) -Degrading Bacterium

Shenqinmycin, which key component is pheazine-1-carboxylic acid (PCA), has a good effect on prevention and cure pimiento epidemic and watermelon wilt diseases, which is broad-spectrum, high efficiency, low toxicity, good environmental compatibility and low cost. During the previous experimentation, it was found that PCA would be degraded easily in field and its protection efficiency was reduced. Therefore, it is important to investigate the bio-degrading pathway to improve the prevention and cure efficiency of Shenqinmycin.The main purpose of this study is to isolate PCA degrading bacteria. In the experiment, PCA is used as sole sources of carbon and energy and a bacterial strain effectively capable of degrading PCA was isolated from pimiento rhizosoil suffered with pimiento epidemic disease. Then, according to morphology, conventional physiological tests, 16S rDNA sequence, and phylogenetic characteristics, it was identified as Sphingomonas sp. Finally, the PCA degradation experiments were conducted both in LB and inorganic salt medium at 220 rpm in 28 oC. The relationship between the bacterial...