Proteomic Approach To The Different Expressed Proteins Induced By Infection Of Xanthomonas Oryzae Pv. Oryzicola In Rice Cultivar Minghui63

Rice bacterial leaf streak (BLS) distributes widely in South China. BLS which was caused by Xanthomonas oryzae pv. oryzicola (Xooc) firstly discovered in the 1950's in Guangdong, and then spread quickly because the infective hybridized rice was popularized. So BLS is one of the major rice diseases in South China and becomes an important subject of quarantine in our country. Presently, the research of rice BLS is only limited in the research of genomics and the transformation of resistant gene, while very few systemic research on the response to BLS at the level of proteins have been reported.So it is greatly significant to investigate the responses proteins to BLS by means of differential proteomics, either in illuminating the molecular mechanism of rice resistance and the control to the express of gene, or the molecular breeding of the rice with high yield, high quality, and high resistance.The materials in this experiment are rice cultivar minghui63 (Oryzae sativa L.) and"89773-1-1", the pathogen Xooc strong pathogenicity in South China. The rice bacterial leaf streak was inoculated to the leaves in six-leaf stage and the leaf proteins were extracted. The defense-response system of rice against Bacterial Leaf Streak was probed into by the analysis of the differentially expressed proteins of Minghui 63 leaf after BLS infection in different inoculation time with the methods of two-dimensional electrophoresis and mass spectrometry.Proteins were extracted from leaves 2, 5, 12, and 48 hours after rice Bacterial Leaf Streak inoculation and separated by two-dimensional (2-D) polyacrylamide gel electrophoresis. 33 proteins resolved on the 2-DE gels were induced or increased in the inoculated leaf. 21 proteins were up-regulated, while 12 proteins were down- regulated. 27 proteins were determined by matrix - assisted laser desorption/ionization-time of flight mass spectrometry, and identified protein database matching. Further analysis of these 27 proteins showed them involved in signal recognition and defense response, including signal transduction proteins, defense associated proteins, metabolism associated proteins and protein synthesis...