Studies Of Cultures Medium And Cultures Modes Of Haematococcus Pluvialis For Enhanced Production Of Astaxanthin

Astaxanthin is the main carotenoid pigment in aquatic animals and is widespread in nature world. The fresh-water green unicellular alga Haematococcus pluvialis (Chlorophyceae) has become a focus due to its ability to accumulate the high-value pigment astaxanthin. It is generally accepted that Haematococcus pluvialis can accumulate the highest levels of astaxanthin (1-3% by dry weight) among the natural creatures and has gained acceptance in aquaculture and other markets as a"concentrated"form of natural astaxanthin. Therefor, the natural carotenoid pigment astaxanthin has become commercially available in aquaculture, poultry feeds and in the worldwide nutraceutical market.A reversed-phase high performance liquid chromatograph (RP-HPLC) method was developed for the separation of astaxanthin ester that had been saponified from the Haematococcus pluvialis. Separation and identification of astaxanthin was completed within 15min.The detection limit and the linear range of astaxanthin was 0.049mg/L and 1-15mg/l, The method was rapid, simple, precite and accurate (precision of the method (RSD) 3.27%, average recovery 100.7%). There is a good linear relationship between the concentration of astaxanthin by HPLC and that by spectrophotometer (y = 8.496x - 12.237, r=0.9991).The medium improving was studied. By the test experimental, the effect of the Vitamin B₁, VitaminB₁2, different species of iron and NaAc on the two culture stage of Haematococcus plvialis was studied. The results showed that: the growth rate and the livability, the static cell ratio, the astaxanthin production under stress condition were improved when the medium (BBM) were enriched with Vitamin B₁, VitaminB₁2 and iron. The medium was improved by adding a few components. When 1g/L NaAc, 0.05ppm VitaminB₁2 and 18μmol/L EDTA-Fe³⁺ were added in the BBM medium, the growth rate can be achieved 0.5 and astaxanthin concentration was 22.58mg/L( 20.43mg/g). The new medium was named CBBM.Semi-continuous cultures were compared with batch culture for the production of...