| The field and pot experiments of different N-application levels on growth and physiological responses and SPS activity of super-rice Yongyou 6(indica-japonica hydrid rice) were counducted in 2005-2006 in comparison with Liangyoupeijiu. Five N-application levels, namely, application 0kgN·hm-2, 90kgN·hm-2, 180kgN·hm-2, 270kgN·hm-2 and 360kgN·hm-2 was applied respectively based on application of 3750kg·hm-2 slikworm litters were designed. The dynamic of tillering growth, dry matter accumulation, SPAD value of leaf, the net photosynthetic rate, sucrose phosphate synthase (SPS) activity and yield formation factor were measured. Results showed:1. The tillering ability of Yongyou 6 was weak, the effect of N-application levels on promotion of tillering of yongyou 6 was significant.2. The effect of N-application on dry matter accumulation of Yongyou 6 was the different from that of Liangyoupeijiu. The gross dry weight of Yongyou 6 in maturity stage was highest when N-application level was 270kgN.hm-2, while Liangyoupeijiu achieved the greatest gross dry weight when N-application level was 180kgN.hm-2.3. The difference of chlorophyll content of leaf is significant among different N-application levels, namely, N24>N18>N12>N6>N0. In addition, Chlorophyll content of Yongyou 6 was lower than that of Lingyoupeijiu.4. The net photosynthetic rate of Yongyou 6 was lower than that of Liangyoupeijiu.5. According to yield response, the optimum N-application level of Yongyou 6 was 270kgN. hm-2, while the optimum N-application level of Liangyoupeijiu was 180kgN.hm-2.6. The determination of SPS activity showed that the SPS activity of flag leaf was higher than that of the top 2nd leaf whether in heading stage or in filling stage. The SPS activity could be regulated by N-application level, especially the effect of N-application levels on SPS activity of flag leaf was significant. The SPS activity in leaf sheath only takes up 36.7% of that in leaf.
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