The Function Analysis Of Maize Transcription Factor ABP2 In Transgenic Rice Under Stress

Abiotic stresses, such as drought, high salinity and low temperature can make plants dehydrated, which eventually leads to rapid increase of reactive oxygen species (ROS). ROS have dual effects on plants: on the one hand, excess ROS cause oxidative stress which results in irreversible injury at both molecular and cellular level; on the other hand, as a signaling molecule, ROS participate in ABA-mediated responses to stresses, including enhancing the expression of antioxidant genes. Dissecting the molecular mechanism underlying the regulation of antioxidant genes is of great importance in improving the performance of plants under stresses.Catalase (CAT) is the main sink of H₂O₂ in plants and plays an indispensable role in stress defence. Transcription factor ABP2 which specifically interacts with an ABA responsive element of maize cat1 has been cloned in our laboratory, and previous studies have demonstrated that ABP2 can activate the expression of reporter gene in both yeast cells and maize suspension cells. The research express that ABP2 can improve stress tolerance.in ArabidopsisThe results obtain through the present research are as follows:1. We recived transgentic rice 6-23-4,19-28-1 and 19-28-13 by PCR method2. The expression of ABP2 were analyzed by Northern .The results showed that ABP2 gene was different expressed in these lines3. The survival results express that transgenticABP2 rice can improve drought tolerance and salt tolerance under drought\salt stress.4. Physiology analysis research proved that transgenticABP2 rice lectrical conductance and MDA was lower than WT ,and relative water content was higher than WT.