Identification And Sequencing Of BAC Clones Come From Five Wild Rice Species That Contain Pi2/9 Locus

The Pi2/9 locus contains at least five known R genes specificities to the fungal pathogen Magnaporthe grisea. They are Pi2, Pi9, Piz-t, Piz, Pigm. The Pi2, Pi9, Piz-t were already cloned by using the map-based cloning strategy. They belong to a gene complex consisting of multiple genes that encode highly homologous nucleotide binding site (NBS) and leucine rich repeat (LRR) proteins.In order to find the genomic dynamics of Pi2/9 locus in wild rice, we first obtained six BAC clones from two genome types (AA and BBCC) by used homologue search. According to southern bloting, We identified two clones that can represent them. After that, we partially sequenced this two BAC clones by using shot-gun strategy.Four BAC clones that contain Pi2/9 locus of Oryza punctata, Oryza officinalis and Oryza minuta were already sequenced. We analysed their sequence, and found that two BAC clones from Oryza punctata and Oryza officinalis can fully corver their Pi2/9 locus.