Studies On Ascosphaera Apis Isozyme And Cluster Analysis Of Bee Chalkbrood Disease

Bee Chalkbrood Disease is a fungal infectious diseases which caused by the Ascosphaera apis and can kill the larvae. Collected from Fujian, Jiangxi, Gansu Province, 10 strains of Ascosphaera apis were isolated and purified. Meanwhile we use the methods of polyacrylamide gel (or starch gel) electrophoresis to research the 10 different strains of Soluble Protein, EST, MDH, LDH, POD, AMY and G-6-PDH. The results show that some of the strains have significantly different isozymes. Soluble protein, and EST have more bands, MDH, LDH, POD bands less, Also LDH, POD bands exist only in one strain. AMY band is not very clear. All the strains have the same bands of G-6-PDH, but not the same concentration. The 10 strains in the analysis show multi-type bands. Using DPS , a statistical analysis software of V8. 01, we conduct the homologous cluster analysis of soluble protein and other six isozymes. It show that different distance coefficient and clustering method can lead to the same results and we divided the ten strains into three group. Combined with bands, we screened a very special strain. Through analysis, we found that the clustering method used in this paper, variable types and the average square deviation are better than others. In addition, the study also explore the method that MDH and EST are dyed on the same piece of gel.