QTLs Mapping Of Genes Resistant To Fusarium Wilt And The Important Characters In Upland Cotton

Xinjiang cotton region plays a hold and balance role in high-quality cotton production areas in China. It has become an obstacle that Fusarium and Verticillium wilt as the major diseases and the fiber qualities of most cultivars in Xinjiang cotton region, which affected high-yield,steady-yield and high-grade in cotton. Selected breeding and planted new cotton varieties are the only solution to the problem. Breeding a new cultivar through the traditional way need a long period, so it doesn't meet the stringent demand of production.Once the molecular markers which closely linked with the important economy traits of cotton were screened, and combined the MAS, we can breed the cultivars with fertility,high-quality and resistance to disease in order to meet the demand in production. This paper constructs the linkage map using cotton cultivars planted and screens the molecular markers which closely linked with the important economy traits of cotton. The conclusions followed:1. T wo genetic linkage maps were constructed using F2 individuals developed by across of Sumian10 with high resistance to Fusarium wilt×Changrong with effecting Fusarium wilt and Sumian12 with high resistance to Fusarium wilt×Anjiran6 with effecting Fusarium wilt. The linkage map comprised 84 loci and 69 loci. These maps were used to map the character of high resistance disease to Fusarium wilt, by composite interval methods. Two QTLs were detected on three linkage groups and seventeen linkage groups, which might mean that the resistance of Fusarium wilt is controlled by one major QTL groups.2. A genetic linkage map was constructed using 124 F2 individuals developed by across of Zms35 with high resistance to Fusarium wilt×Junmian1 with effecting Fusarium wilt. F2 individuals were amplified by 10 SSR primers which were associated with anti disease. One QTL was detected on fifth linkage groups by composite interval methods, which might mean that the resistance of Fusarium wilt is controlled by one major QTL groups. In the linkage group, the marker NAU740 associated with anti disease was tightly linked with 22.0cM interval genetic distance, which was consistent with the previous research. It has been verified that the marker NAU740 was tightly linked with anti disease traits in our laboratory.3. Based on the construction of genetic linkage maps, Using Winqtlcart ten agronomic traits—the First fruit nodes(FFN); Boll number(BN); Boll size(BS); Lint percentage (LP);Seed index (SI); Plant hight(PH);Main-lobe length and width (L1,W1);Second-lobe length and width (L2,W2)—were studied. In Sumian10×Changrong, nine more possible QTLs/loci, which had LOD>2.5, were suggested. In Sumian12×Anjiran6, tweleve more possible QTLs/loci, which had LOD>2.5, were suggested. Based on the construction of genetic linkage maps, Using Winqtlcart the fibre quality properties were studied In Sumian10×Changrong. Four more possible QTLs/loci, which had LOD>2.5, were suggested. The high levels of phenotypic variance explained by the QTLs indicate that these QTLs have large genetic effects, so these QTLs can be used effectively for MAS during the process of breeding in the future.