Study On The Seedling Approach From Vitro Culture In Asparagus(Asparagus Officinalis L.) And Its Embryogensis

Asparagus (Asparagus officinalis L.) is dioecious plants, and it belongs to the family Liliaceae. The male plant growth is prosperous, the yield is high, the anti-disease is strong,the quality is good, the life span is long, The yield is higher than female plant for 20%³0%, therefore cultivating and propagating quickly male plant is the key to improving the high quality and high yield of Asparagus. The plant cell have individual "omnipotent" , passing the male plant leaving culture to keep excellent quality is the shortcut to propagate quickly good plant, especially the plant from embryo has the advantage that they are many, quick of propagating, complete etc. The experiment is passing the different from species and different organ inoculating of Asparagus , point is researching reborn plant path by culturing in vitro , and inducting to come out embryo, explant from plant outside namely influence the way to being plant.From experiment as above, result indicate:1. Caudex segment of Asparagus is cultured to being a seedling has two approach that first it is inducted to adventitious shoots, second way is coming out callus, then growing adventitious shoot. The caudex segment with a bud is well in the culture medium MS+6-BA0.1+NAA0.1, rate of shoot regeneration and num-ber are at the most.The culture medium of strong seedling is MS+6-BA1.0+ NAA0.5,taking root culture me-dium is 1/2MS+NAA0.2+KT0.2+GA0.3, Taking root the rate amounts to 85%, taking root the quantity many, the root is is robust. Rate of shoot regeneration of the green species A2 and A8 are higher the purple spe-cies A3 and All .The middle ten days of March collects plant outside after inoculating is better than May and Jnue,the growth is strong, the color is thick green, rate of shoot regeneration is high.2. Tender leaf and squama of Asparagus is cultured to being a seedling has two approach.. One is inducting adventitious shoots from callus ,the other is inducting embryonal callus from callus, then getting embryo by finishing some stages of somatic embryogenesis. The best of first culture medium is MS+6-BA2.0 +KT0.5+2,4-D1.0, The callus induction ratio is 80%, embryonal callus following culture later, spheroid embryo,pear-shaped embryo are appeared,but being a seedling is few.3 .The anther culture of Asparagus is to being a seedling ,anther is inducted to callus in the hormone of the certain density,callus is cultured in differentiation culture after succeed culture, then embryo appear. T-he anther Callus have the highest induction ratio when the anther were treated at 4℃for four days and in 1 mol·L^-1 sugar solution for 30min.From A1～A10, A3, A5, A7. A8, A9,A10 can be inducted into callus. 2,4-D at induce embryo has important function.After regeneration to culture 15d, spheroid embryo,long-sh aped embryo are appeared.4.The culture in vitro of immature embryo of Asparagus is cultured to being a seedling by the way to embryo almost. It was observed from paraffin sections that the embryoids derived either from the cell or the cell mass on the surface of the callus,from the cell or cell mass in the deep inner part of the callus. They developed gradually onto plantlets via the stage of globular embryo, pear-shaped and cotyledon embryo.