| Bemisia tabaci Gennadius (Homoptera: Aleyrodidae) is a worldwide agriculturalpest. It is an omnivorous insect which has a very broad host range and complicatedhost-preference biotypes from different populations. It is divided into different biotypesaccording to its host ranges, adaptabilities to host plants, capabilities of transmission ofplant virus and genetic variations, and so forth. Therefore, studies on biotypemonitoring and genetic differentiation of Bemisia tabaci may provide scientific basis forits integrated control. The biotypes of Bemisia tabaci are researched with molecularbiological technology in the paper, the main results show that:1. Biotypes of B. tabaci from Jiansu province are studyed employing RAPD andmtDNA -COI marker, the results indicates that B and Q biotype of B. tabaci consist inthe area, and that the latter belongs to the first report in the Jiangsu province.The spreaddirection from south to north in the area of Q biotype of B. tabaci is found duringsuccessive two years (2005 and 2006). Moreover, genetic constructions and gene flowsof two biotypes of different populations of B. tabaci from the southern, middle andnorthern Jiangsu are studyied based on gene sequences of mtDNA-COI. The author findthat B and Q biotype of B. tabaci have 11 and 12 kinds of haplotypes, respectively. Andthat there are some gnetic varvations of different populations of B. tabaci for thesehaplotypes.2. 151 populations of B. tabaci from 8 provinces and municipalities, other thanJiangsu province, in China are analysed employing RAPD marker. The results indicatesthat B and non-B biotype have 93 and 58 populations, respectively. Thereinto, 45poppulations belong to B biotype and 27 pupulations belong to non-B biotype in 72Guangdong populations, 6 poppulations belong to B biotype and 4 pupulations belongto non-B biotype in 10 Guangxi popualtions, 37 poppulations belong to B biotype and18 pupulations belong to non-B biotype in 37 Yunnan populations, 10 poppulationsbelong to B biotype and 3 pupulations belong to non-B biotype in 13 Hainanpopulations, 6 poppulations belong to B biotype and 5 pupulations belong to non-Bbiotype in 11 Guizhou populations, but non-B biotype isn't detected in Henan, Jiangxi and Shanxi popualtion.3. mtDNA-COI gene sequences of 58 non-B biotype populations of B. tabaciwhich their biotypes have been proved by RAPD marker are analysed. The results showthat 6 Yunan populations belong to Q biotype, 20 Guangdong populations and 1Guizhou popualtion belong to ZHJ-2 biotype. Some differences between other 31populations and the known biotypes are found according to mtDNA- COI genesequences.4. Phylogenetic trees are constructed according to mtDNA-COI and 16S rDNAgene sequences of 22 populations of non-B biotype of B.tabaci from Jiangsu,Guangdong, Guangxi, Yunnan, Hainan and Guizhou provinces or and world typicalpopulations, respectively. 22 non-B biotype populations in China are located 4 and 3evolutionary branches in phylogenetic tree based on mtDNA-COI gene sequences and16S rDNA gene sequences, respectively.
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