| Using the improved C-bands technique, we initial established the C-bands karyotype of L.radiata, L.sprengeri, L.chinensis and L.aurea. Using the fluorescence in situ hybridization technique, we located the ribosome sequences, 5S and 45S rDNA in L.sprengeri, L.anhuiensis and L.longitube.The majority of C-bands in four Lycoris genus were telomere bands and intercalary bands. The C-bands karyotype of four Lycoris genus were, L.radiata:2n=3x=33=3CI_+T+3I_++4CI_+T~++8I_+T~++3CT~++3T+3T_++6;L.sprenger:2n=2x=22=2C+ 2I+T~++2T+2T~++4I_+T+2I_+T_++2CI_+T+2I_++4;L.chinensis:2n=2x=16=2CI_+T~++2I_+T_++ 2CT~++4I_++2I~+T~++4; L.aurea:2n=16=A:2CI_+T+4CI_++4CI~+T+2 and B:1CIT~++1I+1C T_++1CI~+T~+.There were equivalent amount bands in L.radiata and L.sprengeri. The quality bands in L.aurea was few than the anterior genus. It was least in L.chinensis. Content of constitutive heterochromatin is, L.sprengeri(17.2%)> L.radiata (16.9%)> L.aurea (16.5%)> L.chinensis(9.42%). In our opinion, the constitutive heteroch- romatin was abridged with the evolutionary in Lycoris genus.Ribosome sequences, 5S and 45S rDNA, were majory distributed in nearly centromere areas. In L.sprengeri, there were six sites of 5S rDNA in distal of chromosomes, and four sites of which were light, while the other two weak; Two sites of 45S rDNA just were detected at chromosome 4 and 5 , which the other homologous ones had no hybridization signals. The result in L.anhuiensis was that six sites of 5S rDNA were detected at chromosome 4,7 and 8; chromosome 4,5,6 and 7 showed eight sites of 45SrDNA, in which the signal at chromosome 4 was most lighteness, and it at chromosome 6 was middle , while the other four signals were weak. There were eight hybridization sites of 5S rDNA distributed in chromosome 4,5,7 and 8 in L.longitube; The chromosome preparation was not so good that eight sites of 45S rDNA could just be located in I chromosomes, while the other one site could not be located.Two probes appeared at the same time in the same place at chromosome 5 and 7 in L.anhuiensis and at least six of I chromosomes in L.longitube. 5S rDNA hybridization sites showed polymorphism in homologous chromosomes 5 of L.anhuiensis and chromosome 4 and 7 of L.longitube. According to the amount of ribosome sequence sites , we principely concluded that the relationship of L.anhuiensis and L.longituba was closer than it of L.sprengeri , and L.anhuiensis was not half higher than L.longituba in evolutionary .On the basis of C-bands, together with fluorescence in situ hybridization(FISH) , we deduced that L.sprengeri was originaler in the evolutionary in 6 Lycoris genus which we studied. L.radiata was evolver than L.sprengeri. The genus which contented the number of chromosomes 16, were later than the anterior genus. It changed largely in chromosomes variance of L.aurea. The relationship was closed between L.sprengeri and L.radiata, L.chinensis and L.aurea, L.anhuiensis and L.longituba. |
