Peanut Transformation Of Sucrose Synthase Gene Mediated By Agrobacterium Tumefaciens

Peanut is an important economical crop and much importance has been attached to its quality improvement through genetic engineering technology. The research transferred potato Sucrose Synthase(SS) gene to peanut plants mediated by Agrobacterium for the further study of the effect of foreign gene expression to the peanut plants'sugar metabolite. It will provide some basis for the selection of peanut transformants with high content of soluble sugars.The cotyledons of mature peanut seeds were used as explants. After Agrobacteria which achieve log stage infect the explants we can transfer them to shoot induction media for co-cultivation. In this stage, high 6-BA /2, 4-D ratio will accelerate the differentiation of the shoots. The rate of the differentiated shoots can come to 30% on average and then cut the strong shoots carefully to shoot elongation media with lower 6-BA inducing the elongation of the shoots. In the next step, we transfer the strong elongated shoots to root induction media without 6-BA for roots. 19 regenerated plants were obtained in all. 5 of them were assessed positive in PCR test about nptâ…¡gene with the transformation rate 0.83%. The sucrose content test shows a 25.6% increasing in leaves of sucrose in one of the 5 plants. The tests of soluble sugar content and SS activity shows that there is no obvious change in the leaves of positive regenerated plants. It tells us, that SS can not increase the soluble sugar content in the peanut plants as a reversible enzyme in the metabolism; and that we have also made some modification in the peanut cotyledon regenerated system mediated by Agrobacterium tumefaciens.