| The goat oocyte took one kind of research experimental material, which waswidely applied in embryo project, the nucleus transplant and the genetransfer.Preservation of oocytes by vitrification, which were not only offer themassive oocytes for the laboratory and be overcome with time and the spatial limit,but also there was a vital significance on protecting the variety resources and IVPcommercialization.This study was conducted to get to the better OPS vitrifcationsolution, enhances the freezing effect of the oocyte. There are six differentvitrifcation solution in this study, which were named vitrifcation solutionONE(30%EG), vitrifcation solution TWO(27.5%EG+2.5%DMSO), vitrifcationsolution THREE (25%EG+5DMSO%), vitrifcation solution FOUR(20%EG+10%DMSO), vitrifcation solution FIVE(15%EG+15%DMSO),vitrifcationsolution SIX(40%EG).The goat IVM oocytes were divided into three groupsaccording to whether they were: (1) left untreated (control); (2) exposed tocryoprotectant agents (the toxicity group); or (3) cryopreserved by theopenpulled-straw (OPS) vitrification method(the vitrification group).oocytes in CPAsand vitrification group were thawed in 0.5mol/Lsucrose and 0.25mol/Lsucrose. In thisstudy,the experiment which we did about the effect of exposure to differentvitrification solution or cryopreservation on the survival of oocyte or thepartheno-activation of oocyte or the fertilization of oocytes and the development ofembryos.In the study on the effect of exposure to different vitrification solution onmortality of oocytes:it was showed that oocytes exposure to different vitrificationsolution, there was no significant differences(P＞0.05)in the rate of complete shapebetween the CPAs control (97.1%,97.0%,97.0%,96.3%,98.1%,96.7%)andcontrol(100.0%); the oocytes which was exposured to in different vitrificationsolution was cultured in SOF for 15-17h,the mortality rate of the vitrification solutionsix (54.1%)was significantly higher(P＜0.05)than the control(0.0%), but there was nosignificant differences (P＞0.05)between other group and the control.so thevitrification solution SIX was eliminated.In the study on the effect of partheno-activation on oocytes exposure to differetvitrification solution:the partheno-activation rate of oocytes which was exposured tovitrification solution FOUR (10%DMSO and 20%EG) or FIVE(EDFS30)wassignificantly higher(P＜0.05)than the controls(0.0%), the partheno-activation rate ofoocytes which was exposured to vitrification solution ONE, TWO, THREE(1.0%,2.7%,3.5%)were not significantly differences(P＞0.05)than the controls(0.0%),so the vitrification solution FOUR, FIVE were eliminated.In the study on the effect of vitrification by different OPS vitrification solutionon the fertilization of oocytes:the fertilization rate (31.8%)of oocytes which were exposed to vitrification solution TWO was not significantly differences(P＞0.05) thanthe controls(51.2%), the fertilization rate(29.2%,29.3%) of oocytes which wereexposed to vitrification solution ONE, THREE were significantly differences(P＜0.05)than the controls(51.2%), the fertilization rate of oocytes which werecryopreserved to vitrification solution TWO was significantly lower(P＜0.05)than thecontrols(51.2%), the fertilization rate of oocytes which were cryopreserved tovitrification solution ONE, THREE were remarkably significantly lower (P＜0.01)thanthe controls (51.2%);by the post-24h cleavage rate/the post-48h cleavage rate forparameter, the cleavage speed (51.9%,50.0%)of oocytes which were cryopreserved tovitrification solution ONE, TWO were significantly slower(P＜0.05)than thecontrols(77.1%),the cleavage speed of oocytes in other groups were remarkablysignificantly slower(P＜0.01)than the controls(77.1%); The results was ZP hardeningthat exposure or vitrification in vitrification solutions on oocytes, dissolutiontime(424.0s～434.9s) of zona pellucida were significantly longer (P＜0.05)for oocytesexposed to vitrification solutions or vitrified in OPS, compared to controls(380.1s).In the study on the effect of vitrification by different OPS vitrification solutionONE, TWO, THREE on the development of IVF embryos: for the IVF embryos, Themorulae rates(37.0%,50.0%,37.5%) of oocytes which were exposed to vitrificationsolution ONE, TWO,THREE were not significantly differences(P＞0.05) than thecontrols(58.3%), while The morulae rates(37.0%,50.0%,37.5%) of oocytes whichwere cryopreserved to vitrification solution ONE, TWO,THREE were significantlydifferences(P＞0.05) than the controls(58.3%), The blastocyst rate of the oocyteswhich was exposed to vitrification solution TWO, THREE (9.4%,9.4%) were notsignificantly differences(P＞0.05) than the controls(27.1%),but The blastocyst rate ofother oocytes were significantly lower (P＜0.05) than the controls(27.1%), Theoocytes were exposed or vitrified in vitrification solutions TWO, it was showed thatafter parthenogenetic activation the cleavage rate(69.2%)and the morulaerates(53.3%) of oocytes in toxicity group were no significantly differences(P＞0.05)than the controls (80.0% and 54.2%),but the cleavage rate (61.4%)and themorulae rates(32.6%)of oocytes in vitrification group were significantlydifferences(P＜0.05) than the controls. The blastocyst rates of oocytes in toxicitygroup (6.7%) showed significant differences (P＜0.05) compared to controls (20.8%),while a remarkably significantly (P＜0.01) reduced percentage of blastocyst (2.3%) byOPS vitrification.
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