| Cancer, heart and blood vessel disease become the most dangerousdiseases to the human health. This is closely related to the metabolismof lipid. To develop a functional factor in lipid that can regulate themetabolism of the lipid is very important. A new functional fattyacid-conjugated linoleic acid (CLA) has been known because itsconspicuous functiohs, like its anticancer effect. It can bewidely usedin the animal faming. The sole oil source can't provide overall fattyacids, so we can supply mixed vegetable oils. At this experiment, the testanimals were three ruminally fistulated Yanbian yellow cattles, thebodyweight was 275±20kg, the project was 4×3 incompletely design, there werefour treatments:controlgroup, group 4％, group 5％and group 6％, oils wereadded to the concentrate at 4％, 5％and 6％levels of the diet (concentratebasis), to detect the effects of mixd vegetable oils on inner environmentof rumen, and to select the best level to use widely. The ratio of roughageand concentrate is 42:58. The composition of the mixed vegetable oils is55％soybean oil, 25％safflower oil and 20％sunflower. The results were asfollows: the pH fell to the lowest level at 3h after feeding, then gotback to the normal level, the changing range is 5.65-7.03, there were nodifferences between the experimental groups and commol group(P＞0.05).The quantities of infusorians inclined to decline because the accessionof the mixed oils, but the difference wasn't significant (P＞0.05). Thecontents of NH3-N and BCP didn't vary markedly (P＞0.05). The contentsof propionate in experimental groups varied markedly (P≤0.0001) atdifferent times, after feeding 3h was the highest point, this wasconsistence in the influence of pH. They were all higher than control group,and group 4％was the highest in the four treatments. The quantities of TVFA and A/P had no differences between the four treatments (P＞0.05). Theaccession of mixed oils increased the contents of UFA in the rumen fluid,the content of C18:1 increased markedly (P=0.0001-0.0005), that meant theraw materials which could use to synthesize CLA increased. The contentsof C18:2 in group 4％were all the highest at each fixed time. The contentOf C18:3 increased as the elevation levels of the cilnin the diets, andat 3h, 6h, 12h, this improved markedly (p=0.003-0.030); The contents ofc9, t11-CLA and t10, c12-CLA in rumen fluid had no differences between thefour treatments. In plasma, C18:0 increased with the addition of oils(P=0.0082-0.022). The contents of C18:1 in experimental groups were allhigher than the control group (P=0.005-0.042); C18:2 and C18:3 allsignificantly increased (P=0.002-0.007; P=0.0001-0.0007), so we couldget that the accession of vegetable oils could increase the contents ofUFA. Contents of C9, t11-CLA were all increased after feeding, thedifference was markedly at 6h(P=0.032). Contents of t10, c12-CLA betweenthe treatments varied markedly(P=0.003-0.011) at 0h, 9h and 12h. Thecontents of total CLA in plasma, group 4％and group 6％were higher thanothers. Because of the accession of vegetable oils, the microbes'productions of unhydrogenation increased. The contents of UFA increasedboth in rumen fluid and in plasma, also increased the contents of CLA.To sum up, the additive level of vegetable oils under 6％didn't havenegative effect on fermentation in rumen. The best additive level in thinexperiment was group 4％for the bodyweight about 275kg of Yambian yellowcattle.
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