Genetic Analysis Of Wheat Genes Conferring Resistance To The Yellow Rust In Wheat-Haynaldia Villosa Translocation Lines And The High-Temperature Resistant Variety Xiaoyan54

Stripe rust, caused by Puccinia striiformis f.sp.tritici, is one of the most serious diseases of wheat in the worldwide, planting resistant cultivars is the most effective and economical methods to control this disease. Using common wheat varieties gene conferring resistance to stripe rust have a very limited role for controlling stipe rust. So many researchers have focused their attention to use of foreign genes to solve this problem. High-temperature resistance to stripe rust is a durable resistance gene that controlled by the major gene and easy selection and use. The genetic analysis and mapping of resistance to stripe rust genes in three wheat-Haynaldia villosa translocation lines and Xiaoyan54 (containing E. elongata blood geographic) were investigated. The results as follow:1. Using the current epidemic yellow rust race CY29 ,CY30,CY31, CY32 and Su4, Su11, Su14 inoculated to seven wheat-Haynaldia villosa translocation lines V9128-1, V9128-3,V9129-1, V3, V4, V5, V12, and the Haynaldia villosa and 7182 for identification of resistance. The results showed: Seven translocation lines resistance to yellow rust race is a marked difference, may be seven resistance genes in translocation lines were vary. We also found that individual translocation lines is not yet stable, it need continue to be self-selection, make resistance gene homozygous stability.2. Genetic analysis of three translocation lines of resistance to stripe rust, the results showed:(1) Single dominant genes determine the reactions of V9128-1 to race CY30.(2) Two dominant genes determine the reactions of V9128-3 to race CY31.(3) One dominant gene and one recessive genes determine the reactions of V3 to race CY31.3. The parents and the resistant and susceptible bulks were used for screening 121 SSR primer combinations .The markers, Xgwm566 and Xgwm376, were tightly linked to YrV1 (temporarily designated).YrV1 was 3.6 cM from Xgwm566, and 5.5 cM from Xgwm376. Therefore, YrV1 was located in the short arm of chromosome 3B. Two markers were tested both the genomic DNA of V9128-1 and the Haynaldia villosa (one of the original parents of V9128-1) .By pedigree and molecular marker analysis of YrV1, the results indicated that YrV1 derived from Haynaldia villosa was likely a novel Yr gene.4. The high-temperature resistant variety Xiaoyan54 expression effective of resistance to stripe rust only after inoculation under high-temperature treatment. But under the high temperature treatment before inoculation of Xiaoyan54 the resistant to stripe rust expression invalid. The results indicated that the high temperature resistance are expressed by the pathogen and temperature induced. The plants,inoculated under high-temperature treatment,were next inoculated under normal tempareture, their resistant did not express . So the high-temperature resistant variety to stripe rust induced in the one-time can not express resistance at all stages of plant growth.5. The race-specific and non - race -specific resistance in Xiaoyan 54 were investigated for the resistance phenotype, classical genetic analysis and molecular markers under high temperature conditions . The results showed : Xiaoyan54 had different infection types ,resistance genes and molecular markers under high temperature conditions,respectively.Its resistant characteristic ought to be believed as race-specific but not with non-race-specific .6. A recessive gene of Xiaoyan 54 for resistance to race CY32 was located on 2A chromosome used a set of Chinese Spring monomer. SSR molecular marker technology was used to detection F2 population that inoculated with race CY31 and CY32. 149 and 169 F2 generation plants were constructed to mapping the high-temperature resistance gene. Linkage analysis showed that Xgwm372 from resistance genes Yrxy54-1 genetic distance was 4.72cM. These two genes are located on 2AL and 7DS, to comparison of known genes in the 2A and 7D chromosome with Yrxy54-1 and Yrxy54-2. The results suggested these two genes are different from known resistance genes to stripe rust. So, that may be to the newly discovered high-temperature resistance genes.7. The identification of resistance in the Chinese Spring seedling and adult plant stage and the genetic analysis of Xiaoyan54×Chinese Spring and Xiaoyan54×Mingxian169 F2 population were investegated under the High- temperature conditions, the results showed that Chinese Spring has high temperature resistance gene at all stages of plant growth and a dominant gene determining Chinese Spring resistance to race CY32.