| Oligosaccharide is a new type of Protective reagent. As an early information member in the plant, it exerts a significant influence upon the invasion of the plant's anti-germ dyes, growth, morphogenesis, etc. In the process of disease-resistant invading dyes ,oligosaccharides can stimulate many kinds of defensive reactions, including the plant protection element synthesis. Because oligosaccharides are non-toxic and won't cause environmental pollution, more and more researchers at home and abroad are doing research on them in recent years, particularly on plant disease management. This article study the different type elicitors which are production of oligosaccharides from Dothiorella gregaria,chitosan and plant cell walls induce Poplar tomentosa callus which was taken as theacceptor material to cause resistance to Poplar Dothiorella gregaria, and the biochemistry mechanism of inducing resistance to Poplar Dothiorella gregaria is further discussed in details.results are as follows,In the first, production of oligosaccharides from Dothiorella gregaria were applicated under acidolysis and the high temperature high pressuredegeneration, different consistency of ethanol (90%,70%,50%) to offer three oligosaccharide elicitors of narrow-range-molecule ( A1, A2 and A3). elicitor B from chitosans with hydroxiding after the acidolysis, elicitors C1, C2 and C3 are extracted from seatangles, orange peels and banana peels. The seven elicitors have activity in the experiment induced.In the second, Poplar tomentosa callus are induced by seven elicitors which can resist Poplar dothiorella gregaria, and we can select three elicitors which have higher induction activeness and the suitable induction density. The highest relative protective impacts on Poplar Dothiorella gregaria are analyzed: 54.8% (elicitor A1 at 20ug/ml), 34.7% (elicitor B at 25ug/ml) and 32.3% (elicitor C3 at 50ug/ml).In the third, are induced by elicitors (A1,B,C3).The result proves elicitors can increase the content of HRGP and chlorogenic acid, the content of HRGP advanced 36.8%～48.3%, the the content of chlorogenic acid increased 97.7%～106.3%. Protective effect is A1>B>C3. HRGP as repairing the decorations of the plant cell wall can strengthen the cell wall anti-penetrability, and plays the physical disease-resistant role. the chlorogenic acid has the toxicity to many kinds of disease germs, and gets up the chemistry disease-resistant function.In the fourth, Poplars tomentosa callus are induced by three elicitors. The result proves elicitors can increase the activity of SOD,CAT,POD,PPO,APX,PAL and chitinase, two days later, Poplar Dothiorella gregaria was inoculated. the enzyme activity displays increasing and decreasing process in certain time.Thier peak of enzyme activity is obviously higher than Comparison.The result indicates three elicitors have the obvious induction to these enzymes.SOD, CAT, POD are important protection enzymes in the plant, and play the vital role which after oxidative burst the active oxygen species are eliminated in the process of the plant and the disease germ. PAL is the disease-resistant metabolism key enzyme in the plant, its active height directly affects the contents of resistant bacteria material (HRGP, chlorogenic acid and plant protection element and so on). Chitinase has the direct destructiveness to the disease fungus cell wall, therefore its active ascension has the obvious disease-resistant function. Elicitors induce the enzymes and affect resist bacteria material , in a certain extent, elicitors are promulgated the biochemistry mechanism of inducing resistance.
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