Study On Anti-TMV Of Secondary Substances From Walnut

Plant virosis is one of the important diseases of crops. This article studied on the anti-virus activities of secondary substances from walnut materials. The results were shown as follows:(1) Based on the chemical pretest and bioassay results, with tobacco mosaic virus (TMV) as test organism, different solvents were used to extract secondary substances from the walnut leaves and green seedcase. The anti-virus activities of extracts were tested. The results indicated that the inhibition activity of the extracts from walnut green seedcase on TMV infection to Nicotiana glutinosa is higher than that the extracts from walnut leaves. The anti-virus components from walnut leaves and green seedcase are mainly components of low and moderate polarity. Using 95% enthanol as solvent, the inhibition activity of extracts is the best and inhibiting rate of extract from walnut green seedcase on TMV was 88.97%. Activities of extracs from different solvents are reduced obviously,the result proved that the hinger inhibiting rate of extracts of 95% ethanol is effect of the synergistic action from many active components.(2) Active tracking method is adopted for the first time to isolate antiviral substanes. Ethyl acetate of the walnut green seedcase is separated into senven parts by chromatography. The anti-virus activities of these parts are tested.Bioassays showed that inhibiting rate of three parts exceeded 50% among them. The P1 had the highest inhibitory rate, which is 71.37%. The inhibiting rate of P2 is 65.43%. The inhibiting rate of P3 is 54.16%.(3) P1, P2 and P4 separated by silica gel column-chromatography all have obvious inhibiting effect. Among all the parts,the inhibiting rate of senven parts exceeded PC-yy when the concentration was at 5 mg·mL-1. There were thress parts which had inhibiting rate over 75%. They are P2-4(83.94%), P1-5(80.56%), P1-6(75.24%), which were main active compound of anti-virus for further separating and purifying.(4) When the concentration was at 2 mg·mL-1, the inhibiting rate over 75% has two parts after P1-5, P1-6 are separated by silica gel column-chromatography. They were P1-5-4(86.00%) and P1-6-2(76.70%), and the P1-5-4 is the main active components of anti-virus.(5) When the concentration was at 1 mg·mL-1, the inhibition of these parts on TMV were all obviously and the inhibiting rate all over 50% after P1-5-4 was separated by silica gel column-chromatography. The best inhibition activities was the P1-5-4-2, and the inhibitory rate is 87.73%. The compound A was isolated and purified from P1-5-4-2 through thin layer chromatography and recrystalization. When the concentration was at 1mg·mL-1, the inhibitory rate of A was 86.92%. The result indicated that the compound A was one of the main antiviral components in acetate extract.