Alalysis Of Milk Production Traits With Microsatellite Markers In Holstein Cattle

This study mensurate production traits of 150 Holstein cattles, and slected 8 microsatellite sites of 7 chromosomes to analysis microsatellite markers with milk production traits by assaying 150 cows from cattle yard of 150 regiment in Shihezi city.The relevances between genetic effect of different site and butter fat,lactoprotein,production of milk were calculated.It was proved that this locus are of hight polymorphism ,the heterozygosity is between 0.58 and 0.86,polymorphic information contents (PIC) is over 0.5. the highest PIC value of BM1443 is 0.84. There are 4 alleles and 8 genotypes in the locus of BM203, and B is superior gene and BB is superior genotype. There are 6 alleles and 8 genotypes in the locus of BM302,and A is superior gene and AD is superior genotype.There are 5 alleles and 7 genotypes in the locus of BM3413,and B is superior gene and BE is superior genotype.There are 6 alleles and 6 genotypes in the locus of UWCA9,and E is superior gene and EE is superior genotype.There are 8 alleles and 9 genotypes in the locus of BM143,and E is superior gene and BD is superior genotype.There are 10 alleles and 8 genotypes in the locus of BM1443,and I is superior gene and CF is superior genotype.There are 7 alleles and 6 genotypes in the locus of BM415,and B is superior gene and BB is superior genotype.It was discovered that microsatellite locus BM302 has remarkable effect on the 305 days milk production by software analysis combining every milk product traits, and AD genotype is more related to this traits. Locus BM143 also has remarkable effect on the 305 milk production,and BH genotype is more related to this traits.BM3413,UWCA9 have high variance analysis valve to milk production.Locus UWCA9 has distinct influence on milk butter fat, and CC is superior genotype. Locus UWCA9 also has insignificant effect on lactoprotein, EF is superior genotype.A method has been established to estimate the production traits of a cow. It use the multiplex PCR technique by select two microsatellite sites which have significant deviaition on 305 days milk production,the ratio of milk butter fat and the lactoprotein.It was proved that it doesn't has significant deviaition between fact value and estimate value. It testified the method to estimate the production traits of a cow is accurate and feasible. The method provide scientific basis for nonage selection on Holstein cattle.