Risk Assessment And Identification Of Fusarium Circinatum

Following the pest analysis norm of International Plant Protection Convention (IPPC) of Food andAgriculture Organization of the United Nations (FAO), in this article the risk of Fusarium circinatumcarrying on wood & wooden packages has been assessed from the geographical and managerial standard,probability of establishment, spread potential, consequences of establishment and entry potential. Theresult demonstrates the risk of the importation into the China of Fusarium circinatum carrying on wood& wooden packages from quarantine area is very high for it up to the geographical and managerialstandard of quarantine pest, the probability of establishment, spread potential, consequences ofestablishment and the entry potential carrying on wood & wooden packages very high in China.Consequently, it is necessary and urgent to take emergency measures to wood & wooden packagesimported into China from quarantine area, at the same time it is in accord with the SPS' principle andinternational practice.The oligonucleotide primers G1/G2 (5'-GCGGTGTCGGTGTGCTTGTA-3'/5'-ACTCACGGCCACCAAACCAC-3'), derived from the differentiation of intergenic spacer (IGS) regions within Fungi,amplified a single 873 bp product from Fusarium spp.. The IGS DNA sequences ofFusarium spp. weregained from GenB ank. Oligonucleotide primers S1/S2(5'-CTTACCTTGGCTCGAGAAGG-3'/5'-CCTACCCTACACCTCTCACT-3') , derived from IGSDNA, amplified a product of 364 bp which was unique to F. circinatum. The nested-PCR using primersG1/G2 and S1/S2 identified F. circinatum from other Fusarium spp.. This PCR assay was proved to behighly sensitive with the detection limit of 5×10^-3 pg.μl^-1 genomic DNA or 10 spores/100μl H₂O. This nested-PCR method can be used to detect Fusarium circinatum from seed, soil and wood directly.