| To investigate the influence of antibiotics usage to the bacterial resistance and the occurrence and developing of the resistant conditioned pathogen, Antibiotics resistance of Enterococcus faecalis in pet hospital, pig farms and chicken farm were studied. These kinds of studies would play an important role on the normative usage of animal medicine and the influence of the resistance transmission from animal to humans. To date, there were no reports on the investigation of resistance of animal source Enterococcus faecalis in our country. The resistance of 554 Enterococcus faecalis isolated from pet hospital, pig farms and chicken farm to 6 kinds of antibiotics were studied with the broth micro-dilution method recommended by The National Committee for Clinical Laboratory Standards(NCCLS). The difference of resistant rate of different original and the influence of the usage of antibiotics to resistance were studied. The result indicated that:The isolated Enterococcus faecalis had higher resistant rate to oxytetracycline and erythromycin. The resistant rate to oxytetracycline from pet hospital, pig farms and chicken farm were 86．5%,99．5%,96．8% respectively and were 72．9%,98．1%,91．4% respectively to etythromycin. The isolation had lower resistant rate to vanoomycin and ampicillin. The resistant rate to vanoomycin from pet hospital, pig farms and chicken farm were 2．1%,3%,3．2% respectively and were 5．2%,6．8%,5．4% respectively to ampicillin. The resistance rate to chloramphenicol and ciprofloxacin in pig farms and chicken farm was similar but it was higher than that of pet hospital. The resistant rate to chloramphenicol from pet hospital, pig farms and chicken farm were 27．1%,70．1%,63．4% and were 22．9%,47．8%,52．7%.The resistant rate of Enterococcus faecalis from three pig farms to six kinds of antibiotics were similar, though these pig farms were quite different in antibiotics usage.The usage of antibiotic to different stages pigs were not the same. So the sample of nursing piglets, weaned pigs, growing pigs, nursing sow, farrowing sow, boar and environment were isolated respectively and nursing piglets, weaned pigs were grouped as young pigs, meanwhile, growing pigs, nursing sow, farrowing sow and boar were grouped as mature pigs. The result indicated that young pigs had higher resistant rate to mature pigs. The resistant rate of young pigs to Oxytetracycline, Chloramphenicol, Vanoomycin, Ampicillin, Ciprofloxacin and Erythromycin were 100％77．4%,4．8%,8．9%,56．5%,100% respectively and were 99．2%,70．4％,2．4％,4%,44．8%,98．4% respectively in mature pigs.The isolation from pig farms and chicken farm had higher resistant level than those from pet hospital. The rate of isolation which has higher MIC value than 128μg /mL from pet hospital, pig farms and chicken farm to oxytetracycline were 36．4%,91．2%,78．5%, and were 2．1%,32．1%,12．6% to Chloramphenicol, were 1%,10%,31．2% to Ciprofloxacin, and were 69．8%,97．5%,83．9% to Erythromycin which were the same to Vanoomycin and Ampicillin.T, L, W, A, H and E were used to represent the resistance to Oxytetracycline, Chloramphenicol, Vanoomycin, Ampicillin, Ciprofloxacin and Erythromycin. The isolation from pet hospital had more TE resistant type and isolation from pig and chicken farms had more TLHE resistant type.The multi-resistance of isolation to the 6 kinds of antibiotics were investigated and the result showed that isolation from pet hospital had more duplicate resistant type, isolation from pig farm had more triplicate resistant type, and isolation from chicken farm had more quadruple resistant type.This study showed the resistant situation of Enterococcus faecalis of animal original in our country, which illustrated the relationship between the usage of antibiotics and the resistant rate and resistant level and multi-resistance.
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