Culturing Of Cysticercus Cellulosae Histiocytes And Establishment Of Their Hybridoma Cells

Cysticercus cellulosae, the larval stage of Taenia solium, is an important parasite which causes neurocysticercosis when encysting in the central nervous system in human or pigs. C. cellulosae can develop into the tapeworm in the intestine when people eat uncooked or poorly cooked cysticercotic pork. The tapeworm carrier as the definitive host is the source of T. solium eggs which are released into the environment with their faeces. T. solium oncospheres can develop into the larval stage in pigs or humans after ingestion of the released eggs. Cysticercosis is a serious public health problem that affects human beings in many developing areas worldwide. The main reason that immunization against cysticercosis is impractical is the limitation of antigens. The primary cells of C. cellulosae have been successfully established in vitro, but their growth velocity is very low. This study therefore has been designed to answer the question above through establishment of new fusion cell stain.Fresh cysticerci were collected from pigs artificially infected with T. solium eggs and the primary cells of C. cellulosae were obtained and cultured in vitro. Their biochemical indexes and micro-structures were investigated. The results showed that they could be passaged up to 20 generations with 14 pair chromosomes. The cell growth cycle 14 to 21 days, the cell line including five kind of cells:the cortical cell cell, the parenchyma cell cell, calcareous bodies cell the flame cell and the sarcoblast;the cell have many kind of shapes:is the ellipse, disc-shaped, the part is the circular, the pear -morph, disc-shaped has the scapula.C. cellulosae cells in good conditions were selected and fused with SP2/0 cells, and positive hybridized cells were identified by ELISA and genetic test. The comparison of the growth curves of the hybridized and primary cells indicated that the growth velocity of the former was higher than that of the latter. In ELISA, the antigens of the hybridized cells were recognized by porcine sera against cysticercosis with 2-times higher values than the control. Moreover mice were immunized by the antigens above and their sera could be detected by native antigens of C. cellulosae. Together with above results suggest that the hybridized cells of C. cellelosae primary cells are established in vitro.