| In this study, we took the genomic unknown sequence of DEV Clone-03, purified by plague assay, as the main subject. We used the partial sequences of the DEV UL30 and UL45 which have been submitted into GenBank as the amplified start point to design the primers were for PCR. 5 086bp (cotained UL31～UL35) and 10 460bp(cotained UL45～UL50) fragments were obtained by the method of targeted gene walking PCR, respectively. Every fragment was sequenced three times in order to determine the propertis of sequence. Sequence analysis revealed that these two fragments contained 12 complete open reading frames (ORFs) which corresponding to the human herpes simplex virus type 1 (HSV-1) UL31, UL32, UL33, UL34, UL35 and UL45, UL46, UL47, UL48, UL49, UL49.5 and UL50, respectively. The ORFs were arranged collinearly with the prototype sequence of herpes simplex virus 1(HSV-1). So we named these 12 gene according to the HSV-1 homologus genes. These two fragments have been submitted into GenBank, the accession number was EF203708 and EF492886.The comparison of nucleic acid and amino acid (aa) sequences of these 12 deduced DEV proteins with other 11 alphaherpesviruses displayed that the high identity relationships with the corrsponding gene. However, the different gene of the DEV Clone-03 showed that identity relationships of nucleic acid and amino acid were not homogenous. So, we can predict that the role of these 12 proteins in the DEV according to the homologous protein. Phylogenetic trees of the putative amino acid encoded by the 12 genes showed that the evolutionary relationship in the Alphaherpesvirinae were not homogenous and most of them (8/12) had a close evolutionary relationship with the Mardivirus in the Alphaherpesvirinae. So we suggested that the DEV should be classified into the genus Mardivirus within subfamily Alphaherpesvirinae in the future. The sequencing of the DEV has a great significancy to the detection, classification and function research for the DEV.
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