Study On The Candidate Gene Determining Growth And Development Difference In Sex-control IVF And In Vivo Embryo

Transferring bovine sex-controlled IVF mbryo usually lead to large offspring syndrome(LOS)which seriously influence the growth and development of fetus. The experiment study on the effect of tranfering sex-controlled IVF embryo and choose the skeletal muscle produced by sex-controlled IVF embryo and AI as the experimental material, choose 4 genes(IGF1,IGF2,myostatin and MHC2a)which are closely related with growth and development in order to study the expression difference of sex-controlled IVF embryo and fetus of AI. The result showed that the female calve percent is 96.5%(335/347), the LOS occur percent is 11.01%(12/103), the pregnancy rate is 34.61%(344/994) after transferring sex-controlled IVF embryo of vitrification. It is obviously higher than pregnancy rate of conventional frozen method 29.18%(P<0.05).The birth weight and chest circumference are significant different (P<0.01)between of sex-controlled IVF embryo and fetus of AI. The expression of IGF1,IGF2,myostatin and MHC2a of sex-controlled IVF embryo and AI embryo have significant changes at different development stages of fetus.The experiment conclusion are follows,1.The birth weight and chest circumference are significant different (P<0.01)between of sex-controlled IVF embryo and fetus of AI.but there is no difference in body length and body height.2.The individual calves body weight is 70kg that blong to LOS.3.The LOS is related to the low pregnancy rate and low survive percent.4.The expression of IGF1,IGF2,myostatin and MHC2a of sex-controlled IVF embryo and AI embryo have significant changes at different development stages of fetus. It is one of the the reasons leading to the large offspring syndrome.