Antiviral Effect Of Alloferon1 And Alloferon2 On Canine Distemper Virus And Canine Parvovirus

Alloferon1 and Alloferon2 were isolated originally from the blood of an experimentally infected blow fly Calliphora vicina, with the following amino acid sequences: H G V S G H G Q H G V H G(Alloferon1) and G V S G H G Q H G V H G(Alloferon2). Previous experiments have revealed that the synthetic Alloferon 1 and Alloferon 2 have stimulatory activities on natural killer cells, induction of IFN production, antiviral and antitumoral effects.To investigate the inhibitory effect of the two antiviral peptides on canine virus replication, different concentrations of Alloferon1 or Alloferon2 were added to the cell culture medium pre-, co- or post-infection and the antiviral effect was observed according to cytopathic effect (CPE). The results showed that both Alloferon 1 and Alloferon 2, pre- or co-added to the cell culture, had inhibitory effect on the replication of canine distemper virus (CDV) and canine parvovirus (CPV) with a dose-dependent effect. The antiviral effect was further evaluated by different route injection of CDV-infected mice, which showed that intracerebral, but not intramuscular injection with Alloferons, had antiviral effect. The antivial effect was further confirmed in chick embryo and unimmunized doges.