Studies On Differential Display Gene Of SST In Chicken

The essential differences between breeds and individuals of poultry are due to the diffrernt gene expressing or the different expressing amount of the same gene. Comparative research on differential expression of genes will obtain the information of life process and uncover the mechanisms of occurrence and development of different traits.A kind of special glandula tubulare named sperm storage tubule (SST) exist in fowl's uterovaginal junction (UVJ), the glandular organ is comprised primarily of tall columnar, non-ciliated, cell nucleus which located at basement of cell line up in order. For the existence of SST, fowl's spermatozoa can survive for long-term, and maintain fertilizability for longer time. Since the discovery of SST, many researches have been done to reveal the histology and reproductive physiology of SST, but the mechanism of sperm storage in SST is still unknow.By using differential display reverse transcription PCR(DDRT-PCR) that piloted by combinations of ten anchor primers and ten random primers, 30 different expressed fragments were obtained, which were cut from polyacrylamide gel electrophoresis products of SST glandular tissue cDNA. With Re-PCR and electrophoresis, 13 different expressed fragments were obtained and 6 ESTs were obtained after cloning and sequencing.By bioinformatics analysis of the 6 ESTs: SST01 is semilar to part of Neurexophilin gene, the homology is 97.8%, presume that SST01 is concerned with the neural regulation of the physiological function; SST02 is semilar to part of MAPKs gene, the homology is 98.7%, presume that SST02 is concerned with epithelial cell proliferation of SST; SST03 is semilar to part of glypican gene, the homology is 98.5, %presume that SST03 is concerned with cell proliferation, differentiation of SST; other 3 ESTs are highly homological to cDNA and dbEST comparing with red jungle fowl in the database of NCBI and dbEST, but function is unknow.Using semiquantitative RT-PCT to test the EST of SST01, SST02 and SST03, the up-regulation expression of these ESTs are concerned with enhancing capability of SST for sperm storage, but the entire sequence function and action mode were unknown, thus further research were needed.This study on the gene level of the hereditary basis of SST provided important information for researching the mechanism of SST for storing sperm and also provided a new method to study on the quality characteristic of New Yangzhou chicken. Further research were needed in the study of the accurate localization of SST gene on chromosome and the structure and functional studies of EST gene.