Effect Of CLA On Proliferation And Differentiation Of Preadipocyte

The conjugated linoleic acid is one kind of new function fat,had the special physiological function.CLA have the anticancer activeness,can cut down the atherosclerosis,adjusts immunity,promote animal growth,suppresses organism fatty deposition,and heavily affects the nutrition assignment,as well as affects the skeleton forming and many kinds of other biological activeness.The present research mainly concentrates in the preadipocyte multiplies and differentiation in the process to regulate the fresh fat process the concrete molecular event and the cell event.Our country just started to the fatty cell research,majority of was about the fatty cell morphology research which not homogeneous was.The experiment explored the culture condition of the preadipocyte in the given lab,on the basis of this,using biology essential method,observed the shape change in preadipocyte differentiation of rat and AA broiler.In this study,the effects of CLA on proliferation and differentiation of preadipocyte is observed by counting cell, examining leptin the adiponectin level in blood,determining OD after Safranin O staining and so on,through adding different dosage of CLA to the preadipocyte.Experimental result as follows:1.The Suitable evaccination density of preadipocyte in this test is rats:4×10~4 and chicken:8×10~4.2.The results of Safranin O staining indicated that,the cell number obviously degraded by CLA treatment.The preadipocyte in CLA treatment group assumes the irregular polygon,the fat drop smaller in the cell,and the cell volume is small.The preadipocyte in control group assumes the circular,the fat drops were bigger in the cell,but the quantity of fat drops is less than that in CLA treatment group.3.The doubling generation time of rats preadipocyte is 65.5h,and the latency period of preadipocyte after vaccination is about 48h,and the preadipocyte increase to 3 times after raised 6d.The 3-9d after preadipocyte vaccination is the doubling generation time,after 9d the growth of preadipocyte enters the balcony period.The latency period of chicken preadipocyte after vaccination is about 48h,and the preadipocyte increase to 3 times after raised 6d.4.The experiment indicated that the CLA be able to degraded significantly the preadipocyte proliferation in rats and chicken.And the different dosage of CLA had the significant deviation. 5.The secretion of leptin and adiponectin in adipocyte of rats and chicken were not striking changed after CLA treatment in this experiment.The effects of CLA on the fatty cell secretion and its possible mechanism of action need further studies.