The Isolation Of Urtica Cannabina L Endophytic Bacteria In XinJiang And Characteristics And Identification Of An Antagonistic Bacteria Against Crop Disease

In order to grope for the the best methods for isolating the endophyte of Urtica cannabina L. and acquire the endophye of Urtica cannabina L., probe into the optimized primer for ERIC-PCR and its value in the application of DNA analysis With AP-PCR, and attain antagonistic endophytic bacteria against pathogenic fungal of economic field crops. In this research, two of the isolation methods were used to isolated the endophyte of Urtica cannabina L., accrording to the number of the bacterial colony, the blendor methods as the best methods for isolating the endophyte of Urtica cannabina L. was decided and the endophyte of Urtica cannabina L. was isolated by using the blendor methods, 12 Strains of endophytic bacteria was screened, According to the observation of the morphology and the result of AP-PCR and ERIC- PCR, these strains were identifyed as Neisse ., Staphylococcus ., Streptococcus., Bacillus., Brevibacterium.s and Enterobacte.s, the electrophoresis strips of modified primer ERIC-PCR fingerprinting were abundant and clear, when ERIC-PCR was used for bacterial typing with AP-PCR,the effect of bacterial typing was enhanced obviously. The antagonistic endophytic bacteria against pathogenic fungal of economic field crops by the method of agar diffusion bioassay, 3 of these endophytic bacteria which have the ability of against the pathogenic fungal of economic field crops was acquired, XJUL-6 against Cotton Verticillium Wilt, XJUL-12 against Watermelon Wilt, XJUL-8 against fusarium wilt of cucumber, the microbiostatin diameter of XJUL-6 is 28mm, the microbiostatin diameter of XJUL-12 is 23mm, the microbiostatin diameter of XJUL-8 is 18mm.According to the observation of the morphology, the study of the physiological and biochemical, 16 SrDNA and G+C mo1% content, the XJUL-6 was identified as Bacillus cereus.,the XJUL-12 was identified as Bacillus subtilis.,XJUL-8 was identified as Bacillus thuringiensis.,At the same time, the biological characters of the bacteria was analyzed primarily, the results of pilot study shows that the optimum temperature range is 36℃~38℃, the optimum pH is pH6~8 and the optimum concentration of NaCl is 2%.