Study On Supplementation Effect Of Controlled-release Bolus On Ruminant

This study is based on the investigation on the deficiency state of these trace elements in soil, water and grazing of Qinghai province and even in ruminants'organism through researching data of papers on this domain, and then to pick out Cu, Zn, Se and Co that are main scanty trace elements in pasture of Qinghai as ingredients of controlled-release bolus. Controlled-release boluses are applied to ruminant animal so that the influence of bolus on the status of MDA, Cu-Zn-SOD and GSH-Px in plasma and the change status of trace elements in blood could be detected, and by the means of that to evaluate the biologic effect of bolus. The test result as followed:1. Taking the boluses out of rumen at 5th, 15th, 30th, 45th, 65th 90th, 120th and 150th day respectively after the degradability test begins, and then according to degraded amount, the degradability curve is draw, that can be studied to make sure the effective period and degradability amount for each day of the bolus. The effective period of the bolus applied on yaks is 90days and 0.0932g for degradability amount for each day. The effective period of the bolus applied on Tibet sheep is 90days and 0.1043g for degradability amount for each day.2. Ruminant animals'plasma or blood is checked to find the change of status of MDA, Cu-Zn-SOD and GSH-Px within it at 5th, 15th, 30th, 45th, 75th, 110th, 150th day and the day before the boluses are applied. The result is that the status of Cu-Zn-SOD and GSH-Px both are higher observably from 30th to 150th day than at the day before the boluses are applied. This higher level of two kind of enzyme reduces the status of MDA in plasma accordingly since 45th day.3. Through applying of boluses in the trial of enhancing weight of ruminants, the positive results are observed: the test teams of ruminant perform better than the comparison teams remarkably. The status of Cu, Zn, Se and Co in ruminant hair at the day that enhancing weight trial finished is remarkably higher than those at the day before boluses were applied, except for the status of Zn in yaks hair and of Co in Tibet sheep hair.